Project 1

Biosensor cell lines for environmental monitoring of drug residues

As tools for environmental monitoring of pharmaceuticals, mode of action (MOA)-based in vitro assays were developed for beta-blockers, as e.g. metoprolol, and non-steroidal anti-inflammatory drugs (NSAIDs), as e.g. diclofenac. For this purpose stable cell lines were generated which expressed recombinant MOA-based sensing and reporting units allowing for rapid live-cell visualization of immediate fluorescence signal changes. Sensitive cell based assays developed in microtiter plate format facilitated the quantitative determination of metoprolol and diclofenac activities in effluents of sewage treatment plant outflows.

Umweltbundesamt publication »From theory to reality – Evaluation of suitable organisms and test systems for the biomitoring of pharmaceuticals«

Two novel real time cell-based assays quantify beta-blocker and NSAID specific effects in effluents of municipal wastewater treatment plants, Water Research, 115, 74-83 (2017)

Project 2

Project 3

Projekt 3: »Biosensor proteins for the development of novel therapeutic approaches for the treatment of cystis fibrosis«

In the frame of the TAT-CF project we developed IGAMI cells. These are inducible fluorescent chloride sensor mCl-EYFP cells that will be used for in vitro screening of potential anionophores that are capable of transporting chloride ions into the cells.

European research and innovation program »tat-cf«